Temporal and geographical lineage dynamics of invasive Streptococcus pyogenes in Australia from 2011 to 2023: a retrospective, multicentre, clinical and genomic epidemiology study.The Lancet. Microbe • April 17, 2024
Ouli Xie, Rebecca Chisholm, Leo Featherstone, An N Nguyen, Andrew Hayes, Magnus Jespersen, Cameron Zachreson, Nefel Tellioglu, Gerry Tonkin Hill, Ravindra Dotel, Stephanie Spring, Alice Liu, Alexander Rofe, Sebastian Duchene, Norelle Sherry, Robert Baird, Vicki Krause, Deborah Holt, Lachlan J Coin, Neela Rai, Matthew O'sullivan, Katherine Bond, Jukka Corander, Benjamin Howden, Tony Korman, Bart Currie, Steven Y Tong, Mark Davies
Background: Defining the temporal dynamics of invasive Streptococcus pyogenes (group A Streptococcus) and differences between hyperendemic and lower-incidence regions provides crucial insights into pathogen evolution and, in turn, informs preventive measures. We aimed to examine the clinical and temporal lineage dynamics of S pyogenes across different disease settings in Australia to improve understanding of drivers of pathogen diversity.
Methods: In this retrospective, multicentre, clinical and genomic epidemiology study, we identified cases of invasive S pyogenes infection from normally sterile sites between Jan 1, 2011, and Feb 28, 2023. Data were collected from five hospital networks across low-incidence regions in temperate southeast Australia and the hyperendemic, tropical, and largely remote Top End of the Northern Territory of Australia. The crude incidence rate ratio (IRR) of bloodstream S pyogenes infection comparing the Top End and southeast Australia and in First Nations people compared with non-First Nations people was estimated by quasi-Poisson regression. We estimated odds ratios (ORs) of intensive care unit (ICU) admission, in-hospital mortality, and 30-day mortality for the Top End versus southeast Australia using logistic regression. Retrieved and successfully sequenced isolates were assigned lineages at whole-genome resolution. Temporal trends in the composition of co-circulating lineages were compared between the two regions. We used an S pyogenes-specific multistrain simulated transmission model to examine the relationship between host population-specific parameters and observed pathogen lineage dynamics. The prevalence of accessory genes (those present in 5-95% of all genomes) was compared across geographies and temporal periods to investigate genomic drivers of diversity.
Results: We identified 500 cases of invasive S pyogenes infection in patients in the Top End and 495 cases in patients in southeast Australia. The crude IRR of bloodstream infection for the Top End compared with southeast Australia was 5·97 (95% CI 4·61-7·73) across the entire study period; in the Top End, infection disproportionately affected First Nations people compared with non-First Nations people (5·41, 4·28-6·89). The odds of in-hospital mortality (OR 0·43, 95% CI 0·26-0·70), 30-day mortality (0·38, 0·23-0·63), and ICU admission (0·42, 0·30-0·59) were lower in the Top End than in southeast Australia. Longitudinal lineage analysis of 642 S pyogenes genomes identified waves of replacement with distinct lineages in the Top End, whereas southeast Australia had a small number of dominant lineages that persisted and cycled in frequency. The transmission model qualitatively reproduced a similar pattern of replacement with distinct lineages when using a high transmission rate, small population size, and high levels of human movement-characteristics similar to those of communities in the hyperendemic Top End. Using a lower transmission rate, larger population size, and lower levels of migration similar to those of communities in urbanised southeast Australia, the transmission model qualitatively reproduced a pattern of dominant lineages that cycled in frequency. Despite distinct circulating lineages, the prevalence of accessory genes in the bacterial population was maintained across geographies and temporal periods.
Conclusions: In a hyperendemic setting, the replacement of distinct S pyogenes lineages occurred in waves, which could be linked to the disproportionate burden of disease and sparse human population in this setting. The maintenance of bacterial gene frequency could be consistent with multilocus selection. These findings suggest that lineage-specific interventions-such as vaccines under development-should consider disease setting and, without broad cross-protection, might lead to lineage replacement. Background: National Health and Medical Research Council, and Leducq Foundation.
Overlapping Streptococcus pyogenes and Streptococcus dysgalactiae subspecies equisimilis household transmission and mobile genetic element exchange.Nature Communications • January 12, 2024
Ouli Xie, Cameron Zachreson, Gerry Tonkin Hill, David Price, Jake Lacey, Jacqueline Morris, Malcolm Mcdonald, Asha Bowen, Philip Giffard, Bart Currie, Jonathan Carapetis, Deborah Holt, Stephen Bentley, Mark Davies, Steven Y Tong
Streptococcus dysgalactiae subspecies equisimilis (SDSE) and Streptococcus pyogenes share skin and throat niches with extensive genomic homology and horizontal gene transfer (HGT) possibly underlying shared disease phenotypes. It is unknown if cross-species transmission interaction occurs. Here, we conduct a genomic analysis of a longitudinal household survey in remote Australian First Nations communities for patterns of cross-species transmission interaction and HGT. Collected from 4547 person-consultations, we analyse 294 SDSE and 315 S. pyogenes genomes. We find SDSE and S. pyogenes transmission intersects extensively among households and show that patterns of co-occurrence and transmission links are consistent with independent transmission without inter-species interference. We identify at least one of three near-identical cross-species mobile genetic elements (MGEs) carrying antimicrobial resistance or streptodornase virulence genes in 55 (19%) SDSE and 23 (7%) S. pyogenes isolates. These findings demonstrate co-circulation of both pathogens and HGT in communities with a high burden of streptococcal disease, supporting a need to integrate SDSE and S. pyogenes surveillance and control efforts.
Australian Society for Parasitology 2023: One Health, one globe.Trends In Parasitology • September 23, 2023
Deborah Holt, Steven Kho, Christian Doerig, Suji O'connor, Madeleine Ray, Maree Widdicombe, Luke Hall, Angelica Tan, Timothy K Ho, Alessia Hysa, Kaitlin Pekin, Keira Brown
Deborah Holt, Steven Kho, Christian Doerig, Suji O'connor, Madeleine Ray, Maree Widdicombe, Luke Hall, Angelica Tan, Timothy K Ho, Alessia Hysa, Kaitlin Pekin, Keira Brown
An outbreak of acute rheumatic fever in a remote Aboriginal community.Australian And New Zealand Journal Of Public Health • January 30, 2023
Natasha Egoroff, Hilary Bloomfield, Wanamula Gondarra, Brando Yambalpal, Terrence Guyula, Demi Forward, Gemma Lyons, Emer O'connor, Lou Sanderson, Michelle Dowden, Desley Williams, Jessica De Dassel, Pasqualina Coffey, Elizabeth Dhurrkay, Veronica Gondarra, Deborah Holt, Vicki Krause, Bart Currie, Kalinda Griffiths, Karen Dempsey, Anna Glynn Robinson
Objective: We describe the public health response to an outbreak of acute rheumatic fever (ARF) in a remote Aboriginal community.
Methods: In August 2021, the Northern Territory Rheumatic Heart Disease Control Program identified an outbreak of acute rheumatic fever in a remote Aboriginal community. A public health response was developed using a modified acute poststreptococcal glomerulonephritis protocol and the National Acute Rheumatic Fever Guideline for Public Health Units.
Results: 12 cases were diagnosed during the outbreak; six-times the average number of cases in the same period in the five years prior (n=1.8). Half (n=6) of the outbreak cases were classified as recurrent episodes with overdue secondary prophylaxis. Contact tracing and screening of 11 households identified 86 close contacts.
Conclusions: This outbreak represented an increase in both first episodes and recurrences of acute rheumatic fever and highlights the critical need for strengthened delivery of acute rheumatic fever secondary prophylaxis, and for improvements to the social determinants of health in the region. Conclusions: Outbreaks of acute rheumatic fever are rare despite continuing high rates of acute rheumatic fever experienced by remote Aboriginal communities. Nevertheless, there can be improvements in the current national public health guidance relating to acute rheumatic fever cluster and outbreak management.
